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Synthesis of Thermogelling Poly(N-isopropylacrylamide)-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering

Article DOI: 10.3791/53704-v • 12:22 min • October 26th, 2016
October 26th, 2016 •
1, 2, 2, 2, 2, 2, 3, 2, 1
1Department of Chemical Engineering, Rowan University, 2Department of Biological Sciences, Rowan University, 3Department of Biomedical Engineering, Drexel University

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An injectable tissue engineering scaffold composed of poly(N-isopropylacrylamide)-graft-chondroitin sulfate (PNIPAAm-g-CS)-containing alginate microparticles was prepared. The adhesive strength, swelling properties and in vitro biocompatibility are analyzed in this study. The characterization techniques developed here may be applicable to other thermogelling systems.

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Thermogelling Poly(N-isopropylacrylamide)-graft-chondroitin Sulfate Composites Alginate Microparticles Tissue Engineering Adhesive Properties Cellular Biocompatibility Hydrogel Composite Intervertebral Disc Tissue Engineering Nucleus Pulposus Thermal Reversible Hydrogels Cell Encapsulation Injectable Thermally-sensitive Composite Tensile Mechanical Tests Cellular Viability Assays Biocompatibility Assays NIPAAm Monomer MCS Purification Deionized Water Purification
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