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Genetics

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JoVE Journal Genetics
Effektiv produktion og identifikation af CRISPR/Cas9-genereret gen Knockouts i Model System Danio rerio
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Effektiv produktion og identifikation af CRISPR/Cas9-genereret gen Knockouts i Model System Danio rerio

Article DOI: 10.3791/56969-v • 11:27 min • August 28th, 2018
August 28th, 2018 •
1, 1, 1
1Department of Biochemistry, Purdue University

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Målrettet genom-redigering i modelsystem Danio rerio (zebrafisk) er blevet stærkt fremmet af fremkomsten af CRISPR-baserede tilgange. Heri, beskriver vi en strømlinet, robust protokol for generation og identifikation af CRISPR-afledte nonsens alleler, der inkorporerer heteroduplex mobilitet analyse og identifikation af mutationer ved hjælp af næste generation sequencing.

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Genetik spørgsmålet 138 Danio reriozebrafisk CRISPR/Cas9 embryo mikroinjektion gen knockout heteroduplex mobilitet assay næste generation sequencing reverse genetik
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