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Journal / Biology / 肠上皮细胞通透性的体外及体内测定方法…
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肠上皮细胞通透性的体外及体内测定方法

Article DOI: 10.3791/57032-v • 10:22 min • October 19th, 2018
October 19th, 2018 •
*1,2, *1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genome Resource Center, Soochow University, 2Department of Oncology, The First Affiliated Hospital of Soochow University
* These authors contributed equally

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本文提出了两种方法来确定肠道屏障功能。在组织培养井中, 使用上皮表 (伏特/欧姆) 直接测量培养的上皮细胞的测定电阻。在小鼠中, 采用 FITC-葡聚糖灌胃法测定体内肠道通透性。

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生物学 问题 140 肠道屏障功能 肠道通透性 测定电阻 FITC-葡聚糖 Caco-2BBe 细胞 灌胃
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