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Análisis cuantitativo de empalmes alternativos del Pre-mRNA en secciones de cerebro de ratón usando análisis de ARN In Situ hibridación

Article DOI: 10.3791/57889-v • 11:22 min • August 26th, 2018
August 26th, 2018 •
1,2, 1,3, 1, 2, 1,3, 1,3,4
1Department of Genetics and Genome Sciences, Case Western Reserve University, 2Department of Endocrinology, Dongfang Hospital of Beijing University of Chinese Medicine, 3Case Comprehensive Cancer Center, Case Western Reserve University, 4Center for RNA Science and Therapeutics, Case Western Reserve University

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Se describe un protocolo en situ hibridación (ISH) que utiliza oligonucleótidos antisentidos corto para detectar patrones de splicing alternativo del pre-mRNA en secciones de cerebro de ratón.

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Genética número 138 hibridación In situ neurofibromatosis tipo 1 exón 23a cerebro de ratón splicing alternativo
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