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In Vivo Mouse Model of Spinal Implant Infection

Article DOI: 10.3791/60560-v • 08:03 min • June 23rd, 2020
June 23rd, 2020 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 2, 3, 2, 2, 2, 2, 2, 1, 1, 1
1Department of Orthopaedic Surgery, University of California Los Angeles, 2David Geffen School of Medicine, University of California Los Angeles, 3University of South Carolina School of Medicine, University of South Carolina

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The protocol describes a novel in vivo mouse model of spinal implant infection where a stainless-steel k-wire implant is infected with bioluminescent Staphylococcus aureus Xen36. Bacterial burden is monitored longitudinally with bioluminescent imaging and confirmed with colony forming unit counts after euthanasia.

Tags

Spinal Implant Infection Mouse Model In Vivo Therapeutics Treatment Strategies Posterior-approach Spinal Surgery Bioluminescent Strain Staphylococcus Aureus Xen36 Bacteria Bioluminescence Imaging Bacterial Burden Peri-implant Tissue Colony Forming Units (CFUs) Animal Models
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