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Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Article DOI: 10.3791/60571-v • 11:17 min • January 18th, 2020
January 18th, 2020 •
1, 2, 1
1Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, 2NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health

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Presented here is a protocol for the generation of a single-cell culture of human embryonic stem cells and their subsequent differentiation into neural progenitor cells. The protocol is simple, robust, scalable, and suitable for drug screening and regenerative medicine applications.

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Neural Differentiation Single-cell Culture Human Embryonic Stem Cells Culture Method Neural Progenitor Cells Neuron Astrocyte Oligodendrocyte Improved Culture System High-density Single Cell Type Culture Molecular Mechanisms Differentiated Lineages Basement Membrane Matrix DMEM/F-12 Feeder-free Cultures
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