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Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy

Article DOI: 10.3791/60849-v • 06:00 min • May 27th, 2022
May 27th, 2022 •
1, 2, 3, 4,5, 4,5, 4,5, 1,6
1University Grenoble Alpes, INSERM UA7, Synchrotron Radiation for Biomedicine (STROBE), 2CNRS, UMR 5254, Université Pau & Pays Adour, E2S UPPA, Institut des Sciences Analytiques et de Physicochimie pour l'Environnement et les Matériaux, 3Institut Jacques Monod, UMR 7592 CNRS, Université Paris Diderot, 4OSUG, UMS 832 CNRS, Université Grenoble Alpes, 5FAME and FAME-UHD beamlines, ESRF, the European Synchrotron, 6ID16A beamline, ESRF, the European Synchrotron

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This protocol presents a detailed procedure to prepare biological cryosamples for synchrotron-based X-ray absorption spectroscopy experiments. We describe all the steps required to optimize sample preparation and cryopreservation with examples of the protocol with cancer and phytoplankton cells. This method provides a universal standard of sample cryo-preparation.

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Biological Samples Preparation Speciation Cryogenic Temperature High-resolution X-ray Absorption Spectroscopy Cellular Specimens Synchrotron Locations Simple Workflow Reliable Preservation Frozen Iterated Cells Pelleting Fast Loading Cryosample Order Unlinking Samples Visual Demonstration Human Prostate And Ovarian Cancer Cell Line Cell Pellets Selenium Speciation T-75 Flasks Cell Culture Medium Laminar Flow Hood Sonicate Nanoparticle Stock Solutions Ultrasound Water Bath Working Concentrations
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