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Neuroscience

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Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology

Article DOI: 10.3791/2032-v • 08:28 min • November 6th, 2010
November 6th, 2010 •
1, 1
1National Eye Institute, National Institutes of Health

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We provide a reproducible method for culturing confluent monolayers of human fetal retinal pigment epithelial cells (hfRPE) cells that exhibit morphology, physiology, polarity, and protein and gene expression patterns of adult native tissue. This work has been extended to an animal model of several eye diseases.

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Retinal Pigment Epithelium Physiology Pathophysiology Cell Culture Procedure Primary Human Fetal Retinal Pigment Epithelium HfRPE Cell Cultures Pigmentation Electron Microscopy Apical Membrane Microvilli Tight Junction Proteins Epithelial Polarity Function Mammalian Models Therapeutic Interventions Animal Models Eye Disease Abnormal Fluid Accumulation Subretinal Space Photoreceptor Outer Segments Retinal Attachments RPE/retina Interactions
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