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Immunology and Infection

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JoVE Journal Immunology and Infection
A Calcium Bioluminescence Assay for Functional Analysis of Mosquito (Aedes aegypti) and Tick (Rhipicephalus microplus) G Protein-coupled Receptors
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A Calcium Bioluminescence Assay for Functional Analysis of Mosquito (Aedes aegypti) and Tick (Rhipicephalus microplus) G Protein-coupled Receptors

Article DOI: 10.3791/2732-v • 10:46 min • April 20th, 2011
April 20th, 2011 •
1, 1, 1,2, 1
1Department of Entomology, Texas A&M University (TAMU), 2Department of Molecular and Cellular Medicine, Texas A&M University (TAMU)

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This protocol provides instructions for clonal-cell line selection and a calcium bioluminescence assay to analyze the structure-activity relationships of synthesized arthropod neuropeptides on their cognate GPCRs. This assay can be used for receptor deorphanization and structure-activity relationship studies for synthetic analog design and peptide/drug-lead discovery.

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Calcium Bioluminescence Assay Functional Analysis Mosquito Aedes Aegypti Tick Rhipicephalus Microplus G Protein-coupled Receptors Arthropod Hormone Receptors Pesticides Kinin Receptors Neuropeptides Structure-activity Relationships Heterologous Kinin Receptor-expressing Systems Biostable Kinin Analogs Diuretic Processes Myotropic Processes Digestive Processes Southern Cattle Tick Boophilus Microplus Aedes Aegypti CHO-K1 Cell Line Calcium Levels Peptide Application Recombinant Cells Aequorin Protein
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