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Immunology and Infection

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A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor

Article DOI: 10.3791/2742-v • 07:45 min • June 27th, 2011
June 27th, 2011 •
1, 1, 1, 1, 2, 3, 3, 1
1Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, 2First department of Internal Medicine, School of Medicine, Kyorin University, 3Neosilk Laboratory, Immuno Biological Laboratories Co., Ltd.

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We designed a cell-free receptor binding assay in order to estimate the binding of granulocyte-macrophage colony-stimulating factor (GM-CSF) to the receptors. It enables us to evaluate competitive inhibition of biotinylated GM-CSF binding to soluble GM-CSF receptor alpha by GM-CSF autoantibody with excellent reproducibility.

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Cell-free Assay System Neutralizing Capacity GM-CSF Antibody Recombinant Soluble GM-CSF Receptor Autoimmune Pulmonary Alveolar Proteinosis (PAP) Disease Severity GM-CSF Bioactivity Neutralizing Capacity Assessment Growth Inhibition Human Bone Marrow Cells TF-1 Cells Bioassay System Cell-free Receptor-binding Assay Transgenic Silkworm Technology Recombinant SGMRα Silk Threads Cocoons
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