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Immunology and Infection

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A Simple Chelex Protocol for DNA Extraction from Anopheles spp.
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A Simple Chelex Protocol for DNA Extraction from Anopheles spp.

Article DOI: 10.3791/3281-v • 07:16 min • January 9th, 2013
January 9th, 2013 •
1, 1, 1, 1, 2, 1, 1
1Malaria Institute at Macha, 2Department of Molecular Microbiology & Immunology, Johns Hopkins Bloomberg School of Public Health

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résumé
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A rapid and affordable way to extract quality malaria parasite and vector DNA from mosquito specimens is described. Capitalizing on chelating properties of Chelex resin, the simple method enables genotyping of malaria parasites in mosquito mid-gut and salivary gland phases, as well as molecular identification of the Anopheles sibling species by PCR.

Tags

DNA Extraction Chelex Protocol Anopheles Spp. Molecular Tools Malaria Parasites Vector Mosquitoes Control Programs Operations Research Field Collected Mosquito Specimens Anopheles Gambiae Sl. Microcentrifuge Tubes Homogenization Deionized Water DNA Extraction Methods Chelex-based Technique Standard Salting Out Extraction
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