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Bioengineering

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Microscopia correlativa per l'analisi strutturale 3D di Interazioni Dinamiche

Article DOI: 10.3791/50386-v • 13:43 min • June 24th, 2013
June 24th, 2013 •
1, 1, 1, 2, 2, 1
1Department of Structural Biology, University of Pittsburgh School of Medicine, 2Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine

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Descriviamo un metodo di microscopia correlativa che combina 3D live-cell microscopia a luce fluorescente ad alta velocità e la tomografia crio-elettroni ad alta risoluzione. Dimostriamo la capacità del metodo correlativo di imaging dinamico, piccoli HIV-1 particelle interagiscono con le cellule HeLa ospitanti.

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Bioingegneria Numero 76 Biologia Molecolare Biologia Strutturale Virologia Biofisica Biologia Cellulare Fisiologia Medicina Ingegneria Biomedica Infezione Microbiologia Tecnologia Industria Agricoltura Scienze della Vita (Generale) la microscopia correlativa CryoET tomografia Cryo-elettrone confocale live-cell imaging microscopia ottica Cryo-fluorescenza HIV-1 capside HeLa cellule cellule virus microscopia imaging
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