Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / gDNA Enrichment by a Transposase-based Technology …
JoVE Journal
Biology

Un abonnement à JoVE est nécessaire pour afficher ce contenu.

JoVE Journal Biology
gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
Histology Basics and Cell Death Detection in Honeybee Tissue
Journal (Biology)
Histology Basics and Cell Death Detection in Honeybee Tissue
Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
Journal (Biology)
Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
 

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Article DOI: 10.3791/51902-v • 08:15 min • October 6th, 2014
October 6th, 2014 •
1, 1
1Department of Biology and Pathology of Tumors, Unit of Molecular Biology, Platform of Immunomonitoring and Genetics, Centre Georges-François Leclerc

Chapitres

résumé
Automatic Translation

gDNA enrichment for NGS sequencing is an easy and powerful tool for the study of constitutional mutations. In this article, we present the procedure to analyse simply the complete sequence of 11 genes involved in DNA damage repair.

Tags

GDNA Enrichment Transposase-based Technology NGS Analysis BRCA1 BRCA2 DNA Damage Repair Next Generation Sequencing Cancer Research Cancer Genetics Genetic Predisposition Nextera Design Study Genes ATM BARD1 BRIP1 CHEK2 PALB2 RAD50 RAD51C RAD80 TP53 Promoter 3'-UTR Patients Transposase Technology Sample Multiplexing Genetic Diagnosis Blood GDNA
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter