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Biochemistry

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Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats
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Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats

Article DOI: 10.3791/54287-v • 12:00 min • September 29th, 2016
September 29th, 2016 •
1, 2, 1
1Department of Oral and Maxillofacial Surgery, University of California, San Francisco, 2Department of Anatomy, University of California, San Francisco

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This manuscript describes how to prepare intact dorsal root ganglia for patch clamp recordings. This preparation maintains the microenvironment for neurons and satellite glial cells, thus avoiding the phenotypic and functional changes seen using dissociated DRG neurons.

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Patch Clamp Recordings Intact Dorsal Root Ganglia Adult Rats Preparation Methods Single Cell Recording Spinal Nerve Stimulation Dorsal Root Stimulation Primary Sensory Neurons Pain Research Neuron And Satellite Glial Cells In Vivo Situation Anesthesia Lumbar Area Shaving Perispinal Muscle Detachment Spinous Process Cutting Vertebral Column Removal ACSF Flushing And Submerging Laminae Removal Dura Mater Cutting Spinal Cord Exposure
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