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Biochemistry

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JoVE Journal Biochemistry
Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
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Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Article DOI: 10.3791/55262-v • 08:13 min • March 4th, 2017
March 4th, 2017 •
1, 1, 1, 1, 1
1Analytical Research & Development, Sanofi Pasteur Limited

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Differential scanning calorimetry measures the thermal transition temperature(s) and total heat energy required to denature a protein. Results obtained are used to assess the thermal stability of protein antigens in vaccine formulations.

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Differential Scanning Calorimetry Thermal Stability Protein Antigen Conformation Molar Heat Capacity Temperature Structural Helicity Fluorophores Thermal Transition Temperature Energy Interaction Stabilizing Tertiary Structure Lot-to-lot Consistency Manufacturing Processes Biologics Visual Demonstration Interactive Medium Differential Scanning Calorimeter Nitrogen Pressure
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