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High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor

Article DOI: 10.3791/57374-v • 09:16 min • June 14th, 2018
June 14th, 2018 •
1,2, 1,2, 2,4, 5, 1, 1, 1, 3, 2, 1, 2
1Department of Coloproctological Surgery, Juntendo University Faculty of Medicine, 2Department of Molecular Pathogenesis, Juntendo University Faculty of Medicine, 3Atopy Research Center, Juntendo University Faculty of Medicine, 4Department of Oral Pathobiological Science and Surgery, Tokyo Dental College, 5Department of Gastroenterology, Juntendo University Faculty of Medicine

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résumé
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To allow highly sensitive detection of the disseminating human colorectal cancer (CRC) cells colonizing tissues, we herein show a protocol for efficient transduction of green fluorescent protein (GFP) lentiviral particles into PDX-derived CRC organoid cells prior to their injection into recipient mice, with stereo-fluorescence microscopic observation.

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High-sensitivity Detection Micrometastases GFP Lentivirus-transduced Organoids Patient-derived Colon Tumor Tumor Biology Colon Cancer Technique Detection GFP-labeled Mice Colon Tumor Organoid-derived Micrometastases Human Colorectal Cancer Cell Organoids Artificial Extracellular Matrix Patient-derived Tumor Xenograft Cell Pellet Colorectal Cancer Organoid Culture Medium Fetal Calf Serum (FCS) Cell Culture Incubator
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