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Biochemistry

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JoVE Journal Biochemistry
Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)
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Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)

Article DOI: 10.3791/58537-v • 10:28 min • November 2nd, 2018
November 2nd, 2018 •
1, 1, 1, 1
1Department of Biochemistry, University of Alberta

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Here, we present a protocol for the production and purification of proteins that are labeled with stable isotopes, and subsequent characterization of protein-protein interactions using Nuclear Magnetic Resonance (NMR) spectroscopy and MicroScale Thermophoresis (MST) experiments.

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Interactions Globular Proteins Filamentous Proteins Nuclear Magnetic Resonance Spectroscopy (NMR) Microscale Thermophoresis (MST) Protein Interaction Field Direct Interactions Atomic Level Air Flow Sample Magnet Spinner Dataset Proton NMR Parameters Solvent Lock Command Resonance Frequency Automatic Tuning Command Wobble Curve Shim Magnetic Field Uniformity
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