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Biochemistry

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JoVE Journal Biochemistry
Nitropeptide profilering og identifisering illustrert av angiotensin II
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Nitropeptide profilering og identifisering illustrert av angiotensin II

Article DOI: 10.3791/59391-v • 07:31 min • June 16th, 2019
June 16th, 2019 •
1,2, 3, 2,4,5,6
1Mass Spectrometry Core Facility, School of Life Sciences, Westlake University, 2Department of Biological Sciences, University of Notre Dame, 3Department of Urology, Shanghai East Hospital, Tongji University School of Medicine, 4Boler-Parseghian Center for Rare and Neglected Diseases, University of Notre Dame, 5Harper Cancer Research Institute, University of Notre Dame, 6Tumor Microenvironment and Metastasis Program, Indiana University Melvin and Bren Simon Cancer Center

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Proteomikk profilering av tyrosin-Nitro proteiner har vært en utfordrende teknikk på grunn av den lave overflod av 3-nitrotyrosine modifikasjon. Her beskriver vi en ny tilnærming for nitropeptide berikelse og profilering ved å bruke angiotensin II som modell. Denne metoden kan utvides for andre in vitro -eller in vivo -systemer.

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Biokjemi Nitropeptide angiotensin II nitrotyrosine dimethyl merking natrium dithionate biotin merking berikelse
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