University of Reims Champagne-Ardenne 5 articles published in JoVE Biology An Innovative 3D-Printed Insert Designed to Enable Straightforward 2D and 3D Cell Cultures Charlie Colin-Pierre1,2,3, Oussama El Baraka3, Laurent Ramont1,2,4, Stéphane Brézillon1,2 1Laboratoire de Biochimie Médicale et Biologie Moléculaire, Université de Reims Champagne-Ardenne, 2UMR CNRS 7369, Matrice Extracellulaire et Dynamique Cellulaire-MEDyC, Université de Reims Champagne-Ardenne Reims, 3BASF Beauty Care Solutions, 4Service Biochimie-Pharmacologie-Toxicologie, Centre Hospitalier Universitaire (CHU) de Reims In this paper, a newly designed 3D-printed insert is presented as a model of co-culture and validated through the study of the paracrine intercellular communication between endothelial cells and keratinocytes. Immunology and Infection Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry Aymric Kisserli1,2, Sandra Audonnet3, Valérie Duret2,4, Thierry Tabary2,4, Jacques Henri Max Cohen2, Rachid Mahmoudi5,6 1Oncogeriatric Coordination Unit, Reims University Hospitals, Maison Blanche Hospital, 2Faculty of Medicine, University of Reims Champagne-Ardenne, 3URCACyt, Flow cytometry technical platform, University of Reims Champagne-Ardenne, 4Department of Immunology, Reims University Hospitals, Robert Debre Hospital, 5Department of Internal Medicine and Geriatrics, Reims University Hospitals, Maison Blanche Hospital, 6Faculty of Medicine, University of Reims Champagne-Ardenne The aim of this method is to determine the CR1 density in the erythrocytes of any subject by comparing with three subjects whose erythrocyte CR1 density is known. The method uses flow cytometry after immunostaining of the subjects' erythrocytes by an anti-CR1 monoclonal antibody coupled to an amplified system using phycoerythrin (PE). Biochemistry Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence Christine Terryn1, Anouck Habrant2, Gabriel Paës2, Corentin Spriet3 1PICT Platform, Université de Reims Champagne-Ardenne, 2Fractionation of AgroResources and Environment (FARE) Laboratory, INRA, Université de Reims Champagne-Ardenne, 3Unité de Glycobiologie Structurale et Fonctionnelle, Université de Lille This protocol describes an original setup combining spectral and fluorescence lifetime measurements to evaluate Förster resonance energy transfer (FRET) between rhodamine-based fluorescent probes and lignin polymer directly in thick plant sections. Chemistry Application of Elemental Lanthanides in the Selective C-F Activation of Trifluoromethylated Benzofulvenes Providing Access to Various Difluoroalkenes Tarun Kumar1,2, Amira Ben Hassine1, Agathe Martinez1, Dominique Harakat1, Sylviane Chevreux1, Fabien Massicot1, Marc Taillefer2, Jean-Bernard Behr1, Jean-Luc Vasse1, Florian Jaroschik1,2 1Institut de Chimie Moléculaire de Reims UMR 7312, Université de Reims Champagne Ardenne, 2Institut Charles Gerhardt Montpellier UMR 5253, Ecole Nationale Supérieure de Chimie de Montpellier This protocol describes the preparation of elemental lanthanides under inert atmosphere and their application in a selective C-F activation process involving trifluoromethylated benzofulvenes. Genetics High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment Aymric Kisserli1,2, Thierry Tabary1,2, Jacques Henri Max Cohen1,2, Valérie Duret1,2, Rachid Mahmoudi3,4 1Department of Immunology, Reims University Hospitals, Robert Debré Hospital, 2Faculty of Medicine, LRN EA 4682, University of Reims Champagne-Ardenne, 3Department of Internal Medicine and Geriatrics, Reims University Hospitals, Maison Blanche Hospital, 4Faculty of Medicine, EA 3797, University of Reims Champagne-Ardenne Here, we describe an innovative method to determine complement receptor 1 (CR1) length polymorphisms for use in several applications, particularly the assessment of susceptibility to diseases such as Alzheimer's disease (AD). This method could be useful to better understand the role of CR1 isoforms in the pathogenesis of AD.