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An Innovative 3D-Printed Insert Designed to Enable Straightforward 2D and 3D Cell Cultures
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JoVE Journal Biology
An Innovative 3D-Printed Insert Designed to Enable Straightforward 2D and 3D Cell Cultures

An Innovative 3D-Printed Insert Designed to Enable Straightforward 2D and 3D Cell Cultures

DOI:
10.3791/64655-v

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08:17 min

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January 06, 2023

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, , ,
1Laboratoire de Biochimie Médicale et Biologie Moléculaire,Université de Reims Champagne-Ardenne, 2UMR CNRS 7369, Matrice Extracellulaire et Dynamique Cellulaire-MEDyC,Université de Reims Champagne-Ardenne Reims, 3BASF Beauty Care Solutions, 4Service Biochimie-Pharmacologie-Toxicologie,Centre Hospitalier Universitaire (CHU) de Reims

Chapters

  • 00:04Introduction
  • 00:57Placement of the Sterilized Insert in the 6‐Well Plates
  • 01:59Seeding of the Cells
  • 04:21Implementation of the Co‐Culture, Cell Observation, Counting, and Scraping
  • 05:12Insert Cleaning for Recycling
  • 05:41Results: Paracrine Intercellular Communication Between Endothelial Cells and Keratinocytes
  • 07:38Conclusion

Summary

Automatic Translation

Automatic Translation

In this paper, a newly designed 3D-printed insert is presented as a model of co-culture and validated through the study of the paracrine intercellular communication between endothelial cells and keratinocytes.

Tags

3D-printed Insert2D Cell Culture3D Cell CultureCell CommunicationCell TypesCell Culture ModelSiliconeKeratinocytesHuman Dermal Microvascular Endothelial CellsCell Culture MediumTrypsinCentrifugation

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