University Hospital Tubingen

7 articles published in JoVE

• Medicine

  
  A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro Wanjing Cen¹, Siegmar Reinert¹, Meltem Avci-Adali², Dorothea Alexander¹, Felix Umrath^{1,3 1}Department of Oral and Maxillofacial Surgery, University Hospital Tübingen, ²Department of Thoracic and Cardiovascular Surgery, University Hospital Tübingen, ³Department of Orthopaedic Surgery, University Hospital Tübingen Here, we present a simple and effective assay procedure for resorption pit assays using calcium phosphate coated cell culture plates.

• Bioengineering

  
  Injection of Porcine Adipose Tissue-Derived Stroma Cells via Waterjet Technology Jasmin Knoll*¹, Marina Danalache*², Walter Linzenbold³, Markus Enderle³, Tanja Abruzzese¹, Arnulf Stenzl¹, Wilhelm K. Aicher^{1 1}Department of Urology, University Hospital Tübingen, ²Department of Orthopaedic Surgery, University Hospital Tübingen, ³ERBE Elektromedizin GmbH Tübingen We present a method of cell injection via needle free waterjet technology coupled with a sequela of post-delivery investigations in terms of cellular viability, proliferation, and elasticity measurements.

• Biology

  
  Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration Florian Christof Bonnaire^1,2, Martina Feierabend³, Julius Michael Wolfgart^1,4, Wolfram Breuer⁵, Christian Walter², Ulf Krister Hofmann^1,2, Marina Danalache^{1 1}Laboratory of Cell Biology, Department of Orthopedic Surgery, University Hospital of Tübingen, ²Department of Orthopedic Surgery, University Hospital of Tübingen, ³Institute for Bioinformatics and Medical Informatics, Faculty of science of the University of Tübingen, ⁴Medical faculty of the University of Tübingen, ⁵Bavarian Health and Food Safety Authority We present a method to investigate spatial chondrocyte organization in the anulus fibrosus of the intervertebral disc using an optical sectioning method.

• Cancer Research

  
  Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia Henrik Landerer*¹, Marlon Arnone*¹, Ronja Wieboldt², Elsa Goersch¹, Anna M. Paczulla Stanger³, Martina Konantz¹, Claudia Lengerke^{1,2,3 1}Department of Biomedicine, University Hospital Basel and University of Basel, ²Division for Hematology, University Hospital Basel and University of Basel, ³Department of Internal Medicine, Hematology and Oncology, University Hospital Tübingen We present two different staining protocols for NKG2D ligand (NKG2DL) detection in human primary acute myeloid leukemia (AML) samples. The first approach is based on a fusion protein, able to recognize all known and potentially yet unknown ligands, while the second protocol relies on the addition of multiple anti-NKG2DL antibodies.

• Bioengineering

  
  Application of Atomic Force Microscopy to Detect Early Osteoarthritis Marina Danalache*¹, Aadhya Tiwari*¹, Viktor Sigwart^1,2, Ulf Krister Hofmann^{1,3 1}Laboratory of Cell Biology, Department of Orthopaedic Surgery, University Hospital of Tübingen, ²Medical faculty of the University of Tübingen, ³Department of Orthopaedic Surgery, University Hospital of Tübingen We present a method to investigate early osteoarthritic changes at the cellular level in articular cartilage by using atomic force microscopy (AFM).

• Medicine

  
  Sucrose Preference and Novelty-Induced Hypophagia Tests in Rats using an Automated Food Intake Monitoring System Martha Anna Schalla*¹, Stephanie Gladys Kühne*¹, Tiemo Friedrich¹, Vivien Hanel¹, Peter Kobelt¹, Miriam Goebel-Stengel^1,2,3, Matthias Rose^1,4, Andreas Stengel^{1,3 1}Charité Center for Internal Medicine and Dermatology, Department for Psychosomatic Medicine, Charité-Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, ²Department of Internal Medicine, Helios Clinic, ³Department of Psychosomatic Medicine and Psychotherapy, University Hospital Tübingen, ⁴Department of Quantitative Health Sciences, University of Massachusetts Medical School Presented here is a protocol to study depression-like and anhedonic behavior in rats. It combines two well-established behavioral methods, the sucrose preference and novelty-induced hypophagia tests, with an automated food and liquid intake monitoring system, to indirectly investigate rodent behavior using surrogate parameters.

• Medicine

  
  Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays Hui Wang^1,2, Anna Paczulla¹, Claudia Lengerke^{1,2 1}Department of Biomedicine, University Hospital Basel, ²Department of Internal Medicine II, University Hospital Tübingen In vitro spheres assays are commonly used to identify cancer stem cells. Here we compare single with multi cell-based spheres assays. The more laborious single cell-based assays or methylcellulose supplementation give more accurate results while multi cell-based assays performed in liquid medium can be highly influenced by cell density.