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Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants
DOI:

08:06 min

October 28, 2022

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Chapters

  • 00:04Introduction
  • 00:42Tissue Processing and Generation of Cartilage Discs
  • 01:14Cartilage Disc Staining and Sorting
  • 02:43Biomechanical Approach of Cartilage Explants
  • 06:11Results: AFM Measurements of Cartilage Discs Based on Cellular Pattern Organization
  • 07:25Conclusion

Summary

Automatic Translation

We present a step-by-step approach to identify and address the most common problems associated with atomic force microscopy micro-indentations. We exemplify the emerging problems on native human articular cartilage explants characterized by various degrees of osteoarthritis-driven degeneration.

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