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Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants
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Bioengineering
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JoVE Journal Bioengineering
Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants

Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants

DOI:
10.3791/64371-v

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08:06 min

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October 28, 2022

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, , ,
1Laboratory of Cell Biology, Department of Orthopaedic Surgery,University Hospital Tübingen, 2Department of Oral and Maxillofacial Surgery,University Hospital Tübingen

Chapters

  • 00:04Introduction
  • 00:42Tissue Processing and Generation of Cartilage Discs
  • 01:14Cartilage Disc Staining and Sorting
  • 02:43Biomechanical Approach of Cartilage Explants
  • 06:11Results: AFM Measurements of Cartilage Discs Based on Cellular Pattern Organization
  • 07:25Conclusion

Summary

Automatic Translation

Automatic Translation

We present a step-by-step approach to identify and address the most common problems associated with atomic force microscopy micro-indentations. We exemplify the emerging problems on native human articular cartilage explants characterized by various degrees of osteoarthritis-driven degeneration.

Tags

Articular CartilageOsteoarthritisMicro-indentationAtomic Force MicroscopyCartilage ExplantsBiomechanical PropertiesIn Vitro ModelCartilage DiscFluorescence MicroscopyAFM Calibration

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