3 articles published in JoVE
Using X-ray Crystallography, Biophysics, and Functional Assays to Determine the Mechanisms Governing T-cell Receptor Recognition of Cancer Antigens Bruce J. MacLachlan*1, Alexander Greenshields-Watson*1, Georgina H Mason*1, Andrea J Schauenburg1, Valentina Bianchi1,2,3, Pierre J Rizkallah1, Andrew K Sewell1, Anna Fuller1, David K Cole1 1Division of Infection and Immunity and Systems Immunity Research Institute, Cardiff University, 2Department of Oncology, University Hospital of Lausanne (CHUV), 3Ludwig Insitutue for Cancer Research, Lausanne Branch, University of Lausanne Here, we describe methods that we commonly employ in the laboratory to determine how the nature of the interaction between the T-cell receptor and tumor antigens, presented by human leukocyte antigens, governs T-cell functionality; these methods include protein production, X-ray crystallography, biophysics, and functional T-cell experiments.
Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond Eleftherios Karanasios1, Eloise Stapleton2, Simon A. Walker1, Maria Manifava1, Nicholas T. Ktistakis1 1Signalling Programme, The Babraham Institute, 2MRC Group, Cardiff School of Biosciences, Cardiff University Time-lapse microscopy of fluorescently labeled autophagy markers allows monitoring of the dynamic autophagy response with high temporal resolution. Using specific autophagy and organelle markers in a combination of 3 different colors, we can follow the contribution of a protein to autophagosome formation in a robust spatial and temporal context.
Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments Xiaoting Meng*1, Wenfei Li*2,3, Fraser Young1, Runchi Gao3, Laura Chalmers3, Min Zhao3, Bing Song1 1School of Dentistry, Cardiff Institute of Tissue Engineering & Repair, Cardiff University, 2Shandong Qianfoshan Hospital, Shandong University School of Medicine, 3Dermatology and Ophthalmology Research, Institute for Regenerative Cures, University of California at Davis This protocol demonstrates methods used to establish 2D and 3D environments in custom-designed electrotactic chambers, which can track cells in vivo/ex vivo using time-lapse recording at the single cell level, in order to investigate galvanotaxis/electrotaxis and other cellular responses to direct current (DC) electric fields (EFs).