3 articles published in JoVE
Fabrication Procedures and Birefringence Measurements for Designing Magnetically Responsive Lanthanide Ion Chelating Phospholipid Assemblies Stéphane Isabettini1, Mirjam E. Baumgartner1, Peter Fischer1, Erich J. Windhab1, Marianne Liebi2, Simon Kuster1 1Laboratory of Food Process Engineering, ETH Zurich, 2MAX IV Laboratory, Lund University Fabrication procedures for highly magnetically responsive lanthanide ion chelating polymolecular assemblies are presented. The magnetic response is dictated by the assembly size, which is tailored by extrusion through nanopore membranes. The assemblies' magnetic alignability and temperature-induced structural changes are monitored by birefringence measurements, a complimentary technique to nuclear magnetic resonance and small angle neutron scattering.
Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue Olaf Bergmann1, Stefan Jovinge1,2 1Strategic Research Center for Stem Cell Biology and Cell Therapy, University of Lund, 2Department of Cardiology Lund University Hospital, University of Lund Cardiac nuclei are isolated via density sedimentation and immunolabeled with antibodies against pericentriolar material 1 (PCM-1) to identify and sort cardiomyocyte nuclei by flow cytometry.
Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney Aaron C. Brown1, Ulrika Blank2, Derek C. Adams1, Michele J. Karolak1, Jennifer L. Fetting1, Beth L. Hill1, Leif Oxburgh1 1Department of Molecular Medicine, Maine Medical Center Research Institute, 2Molecular Medicine and Gene Therapy, Lund University Hospital In this report we describe a method for the isolation and culture of the progenitor cell niche from the embryonic mouse kidney that can be used to study signaling pathways regulating stem/progenitor cells of the developing kidney. These cultured cells are highly accessible to small molecule and recombinant protein treatment, and importantly also to viral transduction, which allows efficient manipulation of candidate pathways.