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An Intra-Tissue Radiometry Microprobe for Measuring Radiance In Situ in Living Tissue
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Bioengineering
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JoVE Journal Bioengineering
An Intra-Tissue Radiometry Microprobe for Measuring Radiance In Situ in Living Tissue

An Intra-Tissue Radiometry Microprobe for Measuring Radiance In Situ in Living Tissue

DOI:
10.3791/64595-v

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09:10 min

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June 02, 2023

•

, ,
1Department of Physics,Yale University, 2Lund Vision Group, Department of Biology,Lund University, 3Department of Physics and Astronomy,University of Pennsylvania, 4Department of Biology,Duke University

Chapters

  • 00:05Introduction
  • 00:53Building the Optical Micro‐Probe
  • 05:09Preparing and Mounting the Tissue for Optical Measurements
  • 06:03Measurement Setup for Tissue Biopsies and Data Collection
  • 08:07Results: Measurement of Radiance Using the Intra‐Tissue Radiometry Probe
  • 08:23Conclusion

Summary

Automatic Translation

Automatic Translation

In this paper, a method for measuring radiance in situ in living tissue is described. This work includes details of the construction of micro-scale probes for different measurements of radiance and irradiance, provides guidance for mounting tissue for the characterization of radiance, and outlines computational methods for analyzing the resulting data.

Tags

Radiance MeasurementSpatially Resolved Spectral MeasurementsAbsorbing MaterialsField LabsGlass SleevePasteur PipetteOptical FiberSMA ConnectorPolyamide Polymer CoatingGlass Fiber

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