2 articles published in JoVE
Fluorescent Labeling of Drosophila Heart Structures Nakissa N. Alayari1,2, Georg Vogler2, Ouarda Taghli-Lamallem2, Karen Ocorr2, Rolf Bodmer2, Anthony Cammarato1,2 1Biology Department, San Diego State University, 2Development and Aging Program, NASCR Center, The Sanford Burnham Institute for Medical Research Here we describe a basic protocol for fluorescent labeling of different elements of heart tubes from larva and adult Drosophila melanogaster. These specimens are well-suited for imaging via fluorescent or confocal microscopy. This technique permits detailed structural analysis of the features of the hearts from a powerful model organism.
Semi-automated Optical Heartbeat Analysis of Small Hearts Karen Ocorr1, Martin Fink2, Anthony Cammarato1,3, Sanford I. Bernstein3, Rolf Bodmer1 1Development and Aging Program, The Sanford Burnham Institute for Medical Research, 2Cardiac Electrophysiology Group, Dept. of Physiology, Anatomy and Genetics, The Sanford Burnham Institute for Medical Research, 3Biology Department and Heart Institute, San Diego State University We have developed a Semi-automated Optical Heartbeat Analysis method (SOHA) for analyzing high speed optical recordings from Drosophila, zebrafish and embryonic mouse hearts. We demonstrate the application of our methodology to the analysis of heart function in fruit fly and embryonic mouse hearts.