3 articles published in JoVE
Exploring Arterial Smooth Muscle Kv7 Potassium Channel Function using Patch Clamp Electrophysiology and Pressure Myography Lioubov I. Brueggemann1, Bharath K. Mani1, Jennifer Haick1, Kenneth L. Byron1 1Department of Molecular Pharmacology & Therapeutics, Loyola University Chicago Measurements of Kv7 (KCNQ) potassium channel activity in isolated arterial myocytes (using patch clamp electrophysiological techniques) in parallel with measurements of constrictor/dilator responses (using pressure myography) can reveal important information about the roles of Kv7 channels in vascular smooth muscle physiology and pharmacology.
A Semi-quantitative Approach to Assess Biofilm Formation Using Wrinkled Colony Development Valerie A. Ray1, Andrew R. Morris1, Karen L. Visick1 1Department of Microbiology and Immunology, Loyola University Medical Center We provide a simple, semi-quantitative method to investigate biofilm formation in vitro. This method takes advantage of the Zeiss stemi 2000-C Dissecting Microscope (with camera attachment) to monitor both the timing and pattern of biofilm formation, as assessed by the development of wrinkled colonies.
Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons Dinesh C. Joshi1, Joanna C. Bakowska1 1Department of Molecular Pharmacology and Experimental Therapeutics, Loyola University Chicago We demonstrate application of the fluorescence indicator, TMRM, in cortical neurons to determine the relative changes in TMRM fluorescence intensity before and after application of a specific stimulus. We also show application of the fluorescence probe H2DCF-DA to assess the relative level of reactive oxygen species in cortical neurons.