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Assay for Blood-brain Barrier Integrity in Drosophila melanogaster
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Assay for Blood-brain Barrier Integrity in Drosophila melanogaster
DOI:

09:08 min

September 18, 2019

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Chapters

  • 00:04Title
  • 00:40Embryo Collection
  • 03:21Larval Collection
  • 04:05Needle Preparation and Specimen Injection
  • 05:43Embryo Imaging
  • 06:17Larval Dissection and Imaging
  • 07:27Results: Representative Blood-Brain Barrier (BBB) Formation Analysis
  • 08:22Conclusion

Summary

Automatic Translation

Blood-brain barrier integrity is critical for nervous system function. In Drosophila melanogaster, the blood-brain barrier is formed by glial cells during late embryogenesis. This protocol describes methods to assay for blood-brain barrier formation and maintenance in D. melanogaster embryos and third instar larvae.

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