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Assay for Blood-brain Barrier Integrity in Drosophila melanogaster
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Chapters
- 00:04Title
- 00:40Embryo Collection
- 03:21Larval Collection
- 04:05Needle Preparation and Specimen Injection
- 05:43Embryo Imaging
- 06:17Larval Dissection and Imaging
- 07:27Results: Representative Blood-Brain Barrier (BBB) Formation Analysis
- 08:22Conclusion
Blood-brain barrier integrity is critical for nervous system function. In Drosophila melanogaster, the blood-brain barrier is formed by glial cells during late embryogenesis. This protocol describes methods to assay for blood-brain barrier formation and maintenance in D. melanogaster embryos and third instar larvae.
