免疫学、問題65、感染症、医学、微生物学、細菌、リアルタイムPCR、プローブ、病原体の検出、血液培養、16S rDNA遺伝子、抗生物質耐性、抗生物質感受性試験 Protocols and Video Articles

Research

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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Authors

Author

Shailesh K. Shahi¹, Kasra Zarei², Natalya V. Guseva¹, Ashutosh K. Mangalam^1,2,3,4

Institution

¹Department of Pathology, University of Iowa, ²Medical Scientist Training Program, University of Iowa, ³Graduate Program in Immunology, University of Iowa, ⁴Graduate Program in Molecular Medicine, University of Iowa

Journal (Biology)

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Authors

Author

Enrico Tatti¹, Boyd A. McKew², Corrine Whitby², Cindy J. Smith¹

Institution

¹Microbiology, School of Natural Sciences, National University of Ireland Galway, ²School of Biological Sciences, University of Essex

Journal (Environment)

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Authors

Author

Sylvie Sanschagrin¹, Etienne Yergeau¹

Institution

¹Energy, Mining and Environment, National Research Council Canada

Journal (Biology)

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Authors

Author

Ayelet Di Segni¹, Tzipi Braun¹, Marina BenShoshan¹, Sarit Farage Barhom¹, Efrat Glick Saar¹, Karen Cesarkas¹, James E. Squires², Nathan Keller^1,3, Yael Haberman^1,4

Institution

¹Sheba Medical Center, ²Children's Hospital of Pittsburgh of UPMC, ³Ariel University, ⁴Cincinnati Children's Hospital Medical Center

Journal (Biology)

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing

Authors

Author

Lisa Couper¹, Andrea Swei¹

Institution

¹Department of Biology, San Francisco State University

Journal (Biology)

A Method for Targeted 16S Sequencing of Human Milk Samples

Authors

Author

Nicole H. Tobin¹, Cora Woodward¹, Sara Zabih¹, David J. Lee¹, Fan Li¹, Grace M. Aldrovandi¹

Institution

¹Department of Pediatrics, University of California at Los Angeles

Journal (Immunology and Infection)

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Education

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16S rRNA Sequencing: A PCR-based Technique to Identify Bacterial Species

Science Education (Advanced Biology)

PCR

JoVE Core (Core: Biologia)

PCR - Polymerase Chain Reaction

JoVE Core (Core: Biologia Molecolare)

Real Time RT-PCR

JoVE Core (Core: Biologia Molecolare)

PCR: The Polymerase Chain Reaction

Science Education (Basic Biology)

RNA Analysis of Environmental Samples Using RT-PCR

Science Education (Environmental Sciences)

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