Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Nucleofection and Primary Culture of Embryonic…
JoVE Journal
Neuroscience

This Contenuto is Open Access.

JoVE Journal Neuroscience
Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons
Portable Thermographic Screening for Detection of Acute Wallenberg's Syndrome
Journal (Neuroscience)
Portable Thermographic Screening for Detection of Acute Wallenberg's Syndrome
A Stainless Protocol for High Quality RNA Isolation from Laser Capture Microdissected Purkinje Cells in the Human Post-Mortem Cerebellum
Journal (Neuroscience)
A Stainless Protocol for High Quality RNA Isolation from Laser Capture Microdissected Purkinje Cells in the Human Post-Mortem Cerebellum
 
Click here for the English version

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

Article DOI: 10.3791/2373-v • 15:40 min • January 24th, 2011
January 24th, 2011 •
*1, *1, *1, 1
1Department of Anatomy, University of Wisconsin-Madison
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

This protocol outlines the steps required to dissect, transfect via electroporation and culture mouse hippocampal and cortical neurons. Short-term cultures may be used for studies of axon outgrowth and guidance, while long-term cultures can be used for studies of synaptogenesis and dendritic spine analysis.

Tags

Nucleofection Primary Culture Embryonic Mouse Hippocampal Neurons Cortical Neurons CNS Neuronal Polarization Axon/dendrite Outgrowth Synapse Formation Glial Conditioning Transfection Electroporation DNA Fluorescently-tagged Fusion Proteins Protein Dynamics Axon Outgrowth Branching Protein Localization CNS Development
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter