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Neuroscience

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JoVE Journal Neuroscience
An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival
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An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival

Article DOI: 10.3791/2685-v • 09:08 min • April 25th, 2011
April 25th, 2011 •
1, 1,2, 1, 1, 1, 1, 1, 1
1National Eye Institute, NIH, 2Ophthalmology Department, The Second Hospital of Harbin Medical University

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This protocol shows how to retrogradely label retinal ganglion cells, and how to subsequently make an optic nerve crush injury in order to analyze retinal ganglion cell survival and apoptosis. It is an experimental disease model for different types of optic neuropathy, including glaucoma.

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Optic Nerve Crush Injury Retinal Ganglion Cell Survival Optic Neuropathy Glaucoma Vision Loss Irreversible Experimental Disease Model Apoptosis Neuronal Death And Survival Therapeutic Measures Therapeutic Reagents Retrograde Labeling Harvest The Retinae RGC Survival
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