An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen

Srinath Govindarajan; Dirk Elewaut; Michael Drennan

doi:10.3791/53256

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Immunologia e infezioni

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Un metodo ottimizzato per l'isolamento ed espansione invariante Natural Killer cellule T da Spleen mouse

An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen

JoVE Journal
Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen

Un metodo ottimizzato per l'isolamento ed espansione invariante Natural Killer cellule T da Spleen mouse

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11,895 Views

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09:01 min

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October 29, 2015

DOI:

10.3791/53256-v

DOI:

10.3791/53256-v

•

09:01 min

October 29, 2015

•

5 Views

Srinath Govindarajan^1,2, Dirk Elewaut^1,2, Michael Drennan^1,2

¹Department of Rheumatology, Laboratory for Molecular Immunology and Inflammation,Ghent University Hospital, ²VIB Inflammation Research Center,Ghent University

Summary

Here we present an adapted protocol that can be used to generate a large number of murine invariant natural killer T cells from mouse spleen. The protocol outlines an approach by which splenic iNKT cells can be enriched for, isolated and expanded in vitro using a limited number of animals and reagents.