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Istituzione di Genome-cura pluripotenti umane Linee di cellule staminali: Da Targeting per isolamento
Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
JoVE Journal
Biologia dello sviluppo
This content is Free Access.
JoVE Journal Biologia dello sviluppo
Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Istituzione di Genome-cura pluripotenti umane Linee di cellule staminali: Da Targeting per isolamento

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09:51 min

February 02, 2016

DOI:

09:51 min
February 02, 2016

13558 Views
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Summary

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Genome editing of human pluripotent stem cells (hPSCs) can be done quickly and efficiently. Presented here is a robust experimental procedure to genetically engineer hPSCs as exemplified by editing the AAVS1 safe harbor locus to express EGFP and introduce antibiotic resistance.

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