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LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism
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LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism
DOI:

06:04 min

April 04, 2016

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Capitoli

  • 00:05Titolo
  • 00:38Preparation of Buffers and 100X Stock Solutions
  • 01:35Platelet Isolation and Performing an Experiment
  • 02:49Quenching and CoA Extraction
  • 04:30Results: Analysis of Human Platelets to Study Mitochondrial Metabolism
  • 05:21Conclusion

Summary

Traduzione automatica

Here we show isolated human platelets can be used as an accessible ex vivo model to study metabolic adaptations in response to the complex I inhibitor rotenone. This approach employs isotopic tracing and relative quantification by liquid chromatography-mass spectrometry and can be applied to a variety of study designs.

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