Espressione transitoria e Localizzazione cellulare di proteine ricombinanti in cellule in coltura per insetti
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April 20, 2017
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Summary
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Nonlytic sistemi di espressione di cellule di insetto sono sottoutilizzate per la produzione, il traffico cellulare / localizzazione, e analisi funzionale proteina ricombinante. Qui, descriviamo metodi per generare vettori di espressione e successiva espressione proteica transiente in cellule lepidotteri disponibili in commercio. La co-localizzazione di Bemisia tabaci acquaporine con proteine marker fluorescenti subcellulare è anche presentato.
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Cite this Article
Fabrick, J. A., Hull, J. J. Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells. J. Vis. Exp. (122), e55756, doi:10.3791/55756 (2017).
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