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Biochemistry

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JoVE Journal Biochemistry
Optimeret protokol til ekstraktion af proteiner fra humanmitralventilen
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Optimeret protokol til ekstraktion af proteiner fra humanmitralventilen

Article DOI: 10.3791/55762-v • 09:13 min • June 14th, 2017
June 14th, 2017 •
1, 2, 1, 1, 1, 1, 2,3,4
1Centro Cardiologico Monzino IRCCS, 2Cardiovascular Tissue Bank of Milan, Centro Cardiologico Monzino IRCCS, 3Department of Clinical Sciences and Community Health, Cardiovascular Section, University of Milan, 4Department of Cardiovascular Disease, Development and Innovation Cardiac Surgery Unit, Centro Cardiologico Monzino IRCCS

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Proteinsammensætningen af ​​den humane mitralventil er stadig delvis ukendt, fordi dens analyse er kompliceret ved lav cellulæritet og derfor ved lav proteinbiosyntese. Dette arbejde tilvejebringer en protokol til effektivt at ekstrahere protein til analyse af mitralventilproteomet.

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Biochemistry human normal mitralventil proteinekstraktion vævshomogenisering proteinanalyse proteomics biokemi
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