Visualizing intracellulare rullante traffico da fluorescenza Lifetime Imaging microscopia
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December 29, 2017
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Summary
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Questo protocollo descrive un nuovo metodo che consente la visualizzazione quantitativa della formazione di complessi di proteine SNARE, basato sul trasferimento di energia per risonanza e la durata di fluorescenza imaging microscopia.
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Cite this Article
Verboogen, D. R. J., Baranov, M. V., ter Beest, M., van den Bogaart, G. Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy. J. Vis. Exp. (130), e56745, doi:10.3791/56745 (2017).
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