Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Identification and Isolation of Oligopotent and…
JoVE Journal
Immunology and Infection

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto.

JoVE Journal Immunology and Infection
Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow
Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo
Journal (Immunology and Infection)
Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo
Preparation of Mouse Pituitary Immunogen for the Induction of Experimental Autoimmune Hypophysitis
Journal (Immunology and Infection)
Preparation of Mouse Pituitary Immunogen for the Induction of Experimental Autoimmune Hypophysitis
 

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

Article DOI: 10.3791/58061-v • 07:21 min • July 29th, 2018
July 29th, 2018 •
1,2, 1,2
1Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, 2Research Division of Immunology, Cedars-Sinai Medical Center

Capitoli

Riepilogo
Automatic Translation

We demonstrate how to identify and isolate 6 subsets of myeloid progenitors from murine bone marrow using a combination of magnetic and fluorescence sorting (MACS and FACS). This protocol can be used for in vitro culture assays (methylcellulose or liquid cultures), in vivo adoptive transfer experiments, and RNA/protein analyses.

Tags

Identification Isolation Oligopotent Lineage-committed Myeloid Progenitors Mouse Bone Marrow Hematologists Immunologists Myeloid Cells Neutrophils Monocytes Dendritic Cells Technique Precise Identification Subsets Strategies Bone Marrow Cells Magnetic-activated Cell Sorting MACS Centrifugation Resuspend MACS Staining Buffer Biotin Antibody Cocktail Incubation Four Degrees Celsius Anti-biotin Microbeads Wash Cells Automated Magnetic Separator Negative Selection
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter