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An Orthotopic Resectional Mouse Model of Pancreatic Cancer

Article DOI: 10.3791/61726-v • 07:17 min • September 24th, 2020
September 24th, 2020 •
1,2,4,5, 1,2, 1,2, 1,2, 3, 1, 1,2, 1,2, 1,2
1Pancreatic Research Group, South Western Sydney Clinical School, University of New South Wales, 2Ingham Institute for Applied Medical Research, 3Centre for Circulating Tumour Cell Diagnostics and Research, Ingham Institute for Applied Medical Research, 4Surgical Innovations Unit, Westmead Hospital, 5Westmead Clinical School, University of Sydney

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In the clinical context, patients with localized pancreatic cancer will undergo pancreatectomy followed by adjuvant treatment. This protocol reported here aims to establish a safe and effective method of modelling this clinical scenario in nude mice, through orthotopic implantation of pancreatic cancer followed by distal pancreatectomy and splenectomy.

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Orthotopic Resectional Mouse Model Pancreatic Cancer Adjuvant Therapy Neoadjuvant Therapy Co-infection Orthotopic Model Pancreatic Resection Technique Immunodeficient Model Immunocompetent Model KPC Tumor Cells Immune Therapies Anesthetized Mouse Sterile Field Povidone Iodine 70% Ethanol Skin Preparation Longitudinal Incision Muscular Layers Forceps Syringe With Cell Suspension Purse String Swab Laparotomy Incision Spleen And Pancreatic Tail Gauze Pancreas Circumferentially
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