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Using the E1A Minigene Tool to Study mRNA Splicing Changes

Article DOI: 10.3791/62181-v • 10:25 min • April 22nd, 2021
April 22nd, 2021 •
1, 1, 1
1Faculdade de Ciências Farmacêuticas, Universidade Estadual de Campinas

Capitoli

Riepilogo
Automatic Translation

This protocol presents a rapid and useful tool for evaluating the role of a protein with uncharacterized function in alternative splicing regulation after chemotherapeutic treatment.

Tags

E1A Minigene Tool MRNA Splicing Changes Alternative Splicing HEK 293 Cells Stable Expression Cell Culturing Transfection DMEM Medium PMTE1A DNA Transfection Reagent Tetracycline Induction Cell Morphology Transfection Efficiency Fluorescent Microscope
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