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Automatisera aggregatkvantifiering i Caenorhabditis elegans
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Automatisera aggregatkvantifiering i Caenorhabditis elegans

Article DOI: 10.3791/62997-v • 07:50 min • October 14th, 2021
October 14th, 2021 •
1, 1, 1, 1,2, 1, 1
1Department of Microbiology and Cell Science, University of Florida, 2Department of Computer and Information Science and Engineering, University of Florida

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Följande protokoll beskriver utvecklingen och optimeringen av ett arbetsflöde med hög genomströmning för maskodling, fluorescensavbildning och automatiserad bildbehandling för att kvantifiera polyglutaminaggregat som en bedömning av förändringar i proteostas.

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Biologi utgåva 176 C. elegans proteostas polyglutamin aggregering
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