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Bioengineering

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JoVE Journal Bioengineering
On-Chip Octanol-Assisted Liposome Assembly for Bioengineering
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On-Chip Octanol-Assisted Liposome Assembly for Bioengineering

Article DOI: 10.3791/65032-v • 09:45 min • March 17th, 2023
March 17th, 2023 •
*1, *1, 1
1Laboratory of Physical Chemistry and Soft Matter, Wageningen University & Research
* These authors contributed equally

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The present protocol describes octanol-assisted liposome assembly (OLA), a microfluidic technique to generate biocompatible liposomes. OLA produces monodispersed, micron-sized liposomes with efficient encapsulation, allowing immediate on-chip experimentation. This protocol is anticipated to be particularly suitable for synthetic biology and synthetic cell research.

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On-Chip Octanol-Assisted Liposome Assembly Bioengineering OLA Microfluidic Platform Synthetic Biology Artificial Cells Liposomes Self-organization Principles High-throughput Manner Encapsulation Efficiency Sample Volumes On-chip Experimentation Biological Reactions Vesicle Dynamics Biomolecular Condensates Membrane Interactions Drug Screening Permeability Testing Antimicrobials Surface Functionalization PV Treatment Octanol Pockets Formation Clean Silicon Wafer
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