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Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation

Article DOI: 10.3791/65131-v • 08:06 min • May 5th, 2023
May 5th, 2023 •
1,2, 1,3, 1,2,3
1Rosalind and Morris Goodman Cancer Research Institute, McGill University, 2Department of Human Genetics, McGill University, 3McGill Integrated Core for Animal Modeling (MICAM), McGill University

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This protocol describes microinjection and in vivo electroporation for regionally restricted CRISPR-mediated genome editing in the mouse oviduct.

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Somatic Genome Engineering Mouse Models In Vivo Microinjection Electroporation Genome Manipulation Oviduct Sporadically Mutated Cells Immunocompetent Environment Cancer Initiation Targeting Organ Mutation Combinations Glass Capillary Tube Sharp Point Snip Dissecting Microscope Sterile Surgery Equipment Microscope Micromanipulator Electroporator Heating Pad Clean Bench Biosafety Cabinet 1X PBS Petri Dish Absorbent Paper Squares
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