RNAi Screening to Identify Postembryonic Phenotypes in <em>C. elegans</em>

Katherine K. Beifuss; Tina L. Gumienny

doi:10.3791/3442

ジャーナル

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生物学

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RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

JoVE Journal
生物学

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JoVE Journal 生物学

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

16,837 Views

•

09:40 min

•

February 13, 2012

DOI:

10.3791/3442-v

DOI:

10.3791/3442-v

•

09:40 min

February 13, 2012

•

10 Views

Katherine K. Beifuss¹, Tina L. Gumienny¹

¹Department of Molecular and Cellular Medicine,Texas A&M University System Health Science Center

概要

We describe a sensitized method to identify postembryonic regulators of protein expression and localization in C. elegans using an RNAi-based genomic screen and an integrated transgene that expresses a functional, fluorescently tagged protein.

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RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

概要

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