JoVE Journal

Neuroscience

JoVE Neuroscience includes methods and techniques for studying the brain and nervous system; also featuring potential treatments for neurological conditions and diseases.

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A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury

Nishanth Lakshman^1,2, Wenjun Xu², Cindi M. Morshead^1,2,3

¹Institute of Medical Science, University of Toronto, ²Department of Surgery, University of Toronto, ³Institute of Biomaterials and Biomedical Engineering, University of Toronto

Isolation of Cerebral Capillaries from Fresh Human Brain Tissue

Anika M.S. Hartz¹, Julia A. Schulz², Brent S. Sokola², Stephanie E. Edelmann¹, Andrew N. Shen¹, Ralf G. Rempe², Yu Zhong¹, Nader El Seblani³, Bjoern Bauer²

¹Sanders-Brown Center on Aging, Department of Pharmacology and Nutritional Sciences, University of Kentucky, ²Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, ³Department of Neuroscience, University of Kentucky

Quantifying Acute Changes in Renal Sympathetic Nerve Activity in Response to Central Nervous System Manipulations in Anesthetized Rats

Anne M. Fink¹, Caron Dean²

¹Department of Biobehavioral Health Science, College of Nursing, University of Illinois at Chicago, ²Department of Anesthesiology, Medical College of Wisconsin and Zablocki VA Medical Center

Operant Protocols for Assessing the Cost-benefit Analysis During Reinforced Decision Making by Rodents

Mojtaba Kermani*^1,3, Zahra Fatahi*², Dechuan Sun³, Abbas Haghparast², Chris French^3,4

¹Department of Optometry and Vision Science, The University of Melbourne, ²Neuroscience Research Center, Shahid Beheshti University of Medical Science, ³Department of Medicine, The University of Melbourne, ⁴Royal Melbourne Hospital

The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning

Cheng Qin*^1,2, Yijiang Bai*^1,2, Zhen Zeng^1,2, Liao Wang^1,2, Zhiwen Luo^1,2, Shunqi Wang^1,3, Suqi Zou^1,3

¹Institute of Life Science, Nanchang University, ²Queen Mary School, Medical Department, Nanchang University, ³School of Life Science, Nanchang University

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Taisuke Yoneda¹, Seiichiro Sakai^1,2, Hisato Maruoka¹, Toshihiko Hosoya¹

¹RIKEN Brain Science Institute, ²Tokyo Metropolitan Institute of Medical Science

Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion

Qiao Li¹, Cheng Qian¹, Feng-Quan Zhou^1,2

¹Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine, ²The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine

Preparation of a High-quality Primary Cell Culture from Fish Pituitaries

Eirill Ager-Wick¹, Kjetil Hodne¹, Romain Fontaine¹, Kristine von Krogh¹, Trude M. Haug², Finn-Arne Weltzien¹

¹Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, ²Department of Oral Biology, Faculty of Dentistry, University of Oslo

Combined Transcranial Magnetic Stimulation and Electroencephalography of the Dorsolateral Prefrontal Cortex

Pantelis Lioumis¹, Reza Zomorrodi¹, Itay Hadas¹, Zafiris J. Daskalakis^1,2, Daniel M. Blumberger^1,2

¹Temerty Centre for Therapeutic Brain Intervention at the Centre for Addiction and Mental Health, ²Department of Psychiatry and Institute of Medical Science, Faculty of Medicine, University of Toronto

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission

Frédéric Knoflach¹, Maria-Clemencia Hernandez¹, Daniel Bertrand²

¹Discovery Neuroscience, Pharma Research and Early Development, Roche Innovation Center Basel, ²HiQScreen Sàrl 6

Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish

Romain Fontaine*¹, Kjetil Hodne*¹, Finn-Arne Weltzien¹

¹Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences

A Rat Model of Central Fatigue Using a Modified Multiple Platform Method

Weiyue Zhang*¹, Wei Zhang*¹, Ning Dai*¹, Chenxia Han², Fengzhi Wu¹, Xu Wang¹, Libo Tan¹, Jie Li¹, Feng Li¹, Qingjia Ren³

¹School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, ²Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, ³Institute of Tibetan Medicine, Tibetan Traditional Medical College

Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains

Kathryn E. Neville¹, Timothy L. Bosse¹, Mia Klekos¹, John F. Mills¹, Marla Tipping¹

¹Department of Biology, Providence College

Where You Cut Matters: A Dissection and Analysis Guide for the Spatial Orientation of the Mouse Retina from Ocular Landmarks

Katelyn B. Sondereker¹, Maureen E. Stabio², Jenna R. Jamil¹, Matthew J. Tarchick¹, Jordan M. Renna¹

¹Department of Biology, The University of Akron, ²Department of Cell and Developmental Biology, University of Colorado Denver

Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ

Jennifer M. Brazill¹, Yi Zhu¹, Chong Li¹, R. Grace Zhai^1,2

¹Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, ²School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University

Investigating Object Representations in the Macaque Dorsal Visual Stream Using Single-unit Recordings

Irene Caprara¹, Peter Janssen^1,2, Maria C. Romero¹

¹Laboratorium voor Neuro-en Psychofysiologie, Katholieke Universiteit Leuven, ²The Leuven Brain Institute

A Volumetric Method for Quantification of Cerebral Vasospasm in a Murine Model of Subarachnoid Hemorrhage

Axel Neulen*¹, Michael Kosterhon*¹, Tobias Pantel*¹, Stefanie Kirschner², Hermann Goetz³, Marc A. Brockmann², Sven R. Kantelhardt¹, Serge C. Thal⁴

¹Department of Neurosurgery, Medical Center of the Johannes Gutenberg - University, ²Department of Neuroradiology, Medical Center of the Johannes Gutenberg - University, ³Platform for Biomaterial Research, Medical Center of the Johannes Gutenberg - University, ⁴Department of Anesthesiology, Medical Center of the Johannes Gutenberg - University

Noninvasive EEG Recordings from Freely Moving Piglets

Nora V. de Camp^1,2, Silke Dietze¹, Markus Klaßen³, Jürgen Bergeler¹

¹Institute of Animal Welfare, Animal Behavior and Laboratory Animal Science, Veterinary Medicine, Free University Berlin, ²Department of Biology, Behavioral Physiology, Humboldt University Berlin, ³Lehr- und Versuchsanstalt für Viehhaltung Hofgut Neumühle

A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate

Fred Yeboah¹, Hongqiu Guo¹, Anke Bill¹

¹Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research

Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains

Shih-Yun Chen¹, Hsiao-Ying Kuo¹, Fu-Chin Liu^1,2

¹Institute of Neuroscience, National Yang-Ming University, ²Brain Research Center, National Yang-Ming University

Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion

Richard B. McCosh¹, Michael J. Kreisman¹, Kellie M. Breen¹

¹Department of Reproductive Medicine, University of California, San Diego School of Medicine

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

Long Mei^1,2, Cheng Zhan², Eric Erquan Zhang²

¹PTN Joint Graduate Program, School of Life Sciences, Peking University, ²National Institute of Biological Sciences, Beijing

Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology

Adel B. Ghnenis¹, Richard E. Czaikowski¹, Zhaojie J. Zhang², Jared S. Bushman¹

¹School of Pharmacy, University of Wyoming, ²Department of Zoology and Physiology, University of Wyoming

Spectral Reflectometric Microscopy on Myelinated Axons In Situ

Junhwan Kwon^1,2, Myunghwan Choi^1,2

¹Department of Biomedical Engineering, Sungkyunkwan University, ²Center for Neuroscience Imaging Research, Institute for Basic Science (IBS)

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