JoVE Journal

Neuroscience

JoVE Neuroscience includes methods and techniques for studying the brain and nervous system; also featuring potential treatments for neurological conditions and diseases.

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Noninvasive EEG Recordings from Freely Moving Piglets

Nora V. de Camp^1,2, Silke Dietze¹, Markus Klaßen³, Jürgen Bergeler¹

¹Institute of Animal Welfare, Animal Behavior and Laboratory Animal Science, Veterinary Medicine, Free University Berlin, ²Department of Biology, Behavioral Physiology, Humboldt University Berlin, ³Lehr- und Versuchsanstalt für Viehhaltung Hofgut Neumühle

A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate

Fred Yeboah¹, Hongqiu Guo¹, Anke Bill¹

¹Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research

Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains

Shih-Yun Chen¹, Hsiao-Ying Kuo¹, Fu-Chin Liu^1,2

¹Institute of Neuroscience, National Yang-Ming University, ²Brain Research Center, National Yang-Ming University

Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion

Richard B. McCosh¹, Michael J. Kreisman¹, Kellie M. Breen¹

¹Department of Reproductive Medicine, University of California, San Diego School of Medicine

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

Long Mei^1,2, Cheng Zhan², Eric Erquan Zhang²

¹PTN Joint Graduate Program, School of Life Sciences, Peking University, ²National Institute of Biological Sciences, Beijing

Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology

Adel B. Ghnenis¹, Richard E. Czaikowski¹, Zhaojie J. Zhang², Jared S. Bushman¹

¹School of Pharmacy, University of Wyoming, ²Department of Zoology and Physiology, University of Wyoming

Spectral Reflectometric Microscopy on Myelinated Axons In Situ

Junhwan Kwon^1,2, Myunghwan Choi^1,2

¹Department of Biomedical Engineering, Sungkyunkwan University, ²Center for Neuroscience Imaging Research, Institute for Basic Science (IBS)

Recording Spatially Restricted Oscillations in the Hippocampus of Behaving Mice

Jonas-Frederic Sauer¹, Michael Strüber¹, Marlene Bartos¹

¹Institute of Physiology I, University of Freiburg

Optogenetic Entrainment of Hippocampal Theta Oscillations in Behaving Mice

Franziska Bender^1,2, Tatiana Korotkova^2,3, Alexey Ponomarenko^1,2

¹Systems Neurophysiology Research Group, Institute of Clinical Neuroscience and Medical Psychology, Medical Faculty, Heinrich Heine University Düsseldorf, ²Behavioural Neurodynamics Group, Leibniz Institute for Molecular Pharmacology (FMP)/ NeuroCure Cluster of Excellence, ³Neuronal Circuits and Behavior Research Group, Max Planck Institute for Metabolism Research

Optogenetics Identification of a Neuronal Type with a Glass Optrode in Awake Mice

Munenori Ono¹, Shinji Muramoto¹, Lanlan Ma¹, Nobuo Kato¹

¹Department of Physiology, Kanazawa Medical University

An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins

Donatus Riemann¹, Andoniya Petkova¹, Thomas Dresbach¹, Rebecca Wallrafen¹

¹Institute for Anatomy and Embryology, University Medical Centre Göttingen

Exogenous Administration of Microsomes-associated Alpha-synuclein Aggregates to Primary Neurons As a Powerful Cell Model of Fibrils Formation

Giulia Panattoni^1,2, Lucia Rota¹, Emanuela Colla¹

¹Bio@SNS Laboratory, Scuola Normale Superiore, ²International School for Advanced Studies (SISSA/ISAS)

A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae

Jiangqu Liu*¹, Takaaki Sokabe*^2,3, Craig Montell¹

¹Neuroscience Research Institute and Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, ²Division of Cell Signaling, National Institute for Physiological Sciences, National Institutes of Natural Sciences, ³Thermal Biology Group, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences

Single Sensillum Recordings for Locust Palp Sensilla Basiconica

Hongwei Li¹, Yinwei You², Long Zhang¹

¹Department of Entomology, China Agricultural University, ²Bio-tech Research Center, Shandong Academy of Agricultural Sciences

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Gabrielle Devienne*¹, Benjamin Le Gac*¹, Juliette Piquet*¹, Bruno Cauli¹

¹UPMC Univ Paris 06, INSERM, CNRS, Neuroscience Paris Seine - Institut de Biologie Paris Seine (NPS - IBPS), Sorbonne Université

A Protocol for Measuring Cue Reactivity in a Rat Model of Cocaine Use Disorder

Andrea L. Dimet*^1,2,3, Irma E. Cisneros*^1,4,5, Robert G. Fox^1,6, Sonja J. Stutz^1,6, Noelle C. Anastasio^1,6, Kathryn A. Cunningham^1,6, Kelly T. Dineley^1,3,4

¹Center for Addiction Research, University of Texas Medical Branch, ²Institute for Translational Sciences, University of Texas Medical Branch, ³Mitchell Center for Neurodegenerative Diseases, University of Texas Medical Branch, ⁴Department of Neurology, University of Texas Medical Branch, ⁵Department of Pathology, University of Texas Medical Branch, ⁶Department of Pharmacology and Toxicology, University of Texas Medical Branch

In Vivo Electrophysiological Measurement of Compound Muscle Action Potential from the Forelimbs in Mouse Models of Motor Neuron Degeneration

Eveliina Pollari^1,2, Robert Prior^1,2, Wim Robberecht^1,2,3, Philip Van Damme^1,2,3, Ludo Van Den Bosch^1,2

¹Department of Neurosciences, Experimental Neurology, KU Leuven - University of Leuven, ²Center for Brain & Disease Research, Laboratory of Neurobiology, VIB, ³Department of Neurology, University Hospitals Leuven

Microstate and Omega Complexity Analyses of the Resting-state Electroencephalography

Fei Gao¹, Huibin Jia², Yi Feng¹

¹Department of Pain Medicine, Peking University People's Hospital, ²Key Laboratory of Child Development and Learning Science of Ministry of Education, Research Center for Learning Science, School of Biological Sciences & Medical Engineering, Southeast University

Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue

Aaron A. May-Zhang¹, Karen K Deal¹, E. Michelle Southard-Smith¹

¹Vanderbilt University Medical Center

Pentylenetetrazole-Induced Kindling Mouse Model

Tadayuki Shimada¹, Kanato Yamagata¹

¹Synaptic Plasticity Project, Tokyo Metropolitan Institute of Medical Science

Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains

Giulia Quattrocolo¹, Maria Isaac², Yajun Zhang², Timothy J. Petros²

¹Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, ²Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health

CMAP Scan MUNE (MScan) - A Novel Motor Unit Number Estimation (MUNE) Method

Anna B. Jacobsen¹, Hugh Bostock², Hatice Tankisi¹

¹Department of Clinical Neurophysiology, Aarhus University Hospital, ²Institute of Neurology, Queen Square House

Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ

Kimberly Young¹, Helena Morrison¹

¹College of Nursing, University of Arizona

An Invasive Method for the Activation of the Mouse Dentate Gyrus by High-frequency Stimulation

Zhe Zhao¹, Haitao Wu^1,2

¹Department of Neurobiology, Beijing Institute of Basic Medical Sciences, ²Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University

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