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Dermatitis, Allergic Contact: A contact dermatitis due to allergic sensitization to various substances. These substances subsequently produce inflammatory reactions in the skin of those who have acquired hypersensitivity to them as a result of prior exposure.

Effects of Exposure of Formaldehyde to a Rat Model of Atopic Dermatitis Induced by Neonatal Capsaicin Treatment

1Neuroscience Research Institute & Department of Physiology, College of Medicine, Korea University, 2Department of Pharmaceutics and Biotechnology, College of Medical Engineering, Konyang University, 3Division of Biological Science and Technology, Science and Technology College, Yonsei University Wonju Campus

JoVE 55987


 Immunology and Infection

Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent

1Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, Eberhard Karls University of Tübingen, 2Department of Nuclear Medicine, Eberhard Karls University of Tübingen, 3Department of Dermatology, Eberhard Karls University of Tübingen

JoVE 55180


 Immunology and Infection

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

1Department of Diagnostic and Interventional Radiology, University Medical Center, Hamburg, 2Institute of Immunology, University Medical Center, Hamburg, 3University Cancer Center Hamburg, University Medical Center, Hamburg, 4Department of Oncology and Hematology, University Medical Center, Hamburg

JoVE 52462


 Medicine

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A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

1State Key Laboratory of Respiratory Disease, 2National Clinical Research Center of Respiratory Disease, 3Guangzhou Institute of Respiratory Diseases, 4Department of Allergy and Clinical Immunology, First Affiliated Hospital of Guangzhou Medical University, 5Department of Respiratory Medicine, Sixth Affiliated Hospital of Guangzhou Medical University

JoVE 55723


 Medicine

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Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-Cut Lung Slices

1Fraunhofer Institute for Toxicology and Experimental Medicine, Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 2Institute for Pathology, Hannover Medical School, Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 3Division of Thoracic and Vascular Surgery, Klinikum Region Hannover (KRH), 4Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 5Institute of Pharmacology and Toxicology, RWTH Aachen University, 6Institute for Immunology, Hannover Medical School, Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH)

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JoVE 57042


 JoVE In-Press

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Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

1Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, 2Division of Allergy and Immunology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University

JoVE 52505


 Immunology and Infection

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Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs

1Program for the Study and Control of Tropical Diseases -PECET-School of Medicine, University of Antioquia, 2School of Agrarian Sciences, University of Antioquia

JoVE 3533


 Immunology and Infection

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Determining Immune System Suppression versus CNS Protection for Pharmacological Interventions in Autoimmune Demyelination

1Physical Medicine and Rehabilitation, University of Alabama at Birmingham, 2Department of Pathology, University of Alabama at Birmingham, 3Department of Neurobiology, University of Alabama at Birmingham, 4Center for Glial Biology and Medicine, University of Alabama at Birmingham

JoVE 54348


 Immunology and Infection

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Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

1Department of Molecular Biology, University of Salzburg, 2Department of Neurology, Paracelsus Medical University, 3Department of Dermatology, Paracelsus Medical University, 4Bühlmann Laboratories, 5Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg

JoVE 3263


 Immunology and Infection

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Preparing and Administering Intramuscular Injections

JoVE 10261

Source: Madeline Lassche, MSNEd, RN and Katie Baraki, MSN, RN, College of Nursing, University of Utah, UT

Intramuscular (IM) injections deposit medications deep into the muscle tissue. Since muscle fibers are well perfused, this route of administration provides quick uptake of the medication and allows for the administration of relatively large volumes. Skeletal muscles have fewer pain-sensing nerves than subcutaneous tissue, which allows for the less painful administration of irritating drugs (e.g., chlorpromazine, an anti-psychotic). IM injections are recommended for patients unable to take oral medications and for uncooperative patients. Some examples of medications that are commonly delivered by IM injections include antibiotics, hormones, and vaccinations. As in any other route of administration, the nurse must consider if the medication is appropriate, given the patient's medical conditions, allergies, and current clinical status. In addition, specifically for IM injections, it is important to assess the patient's muscle mass to determine the appropriate needle size. Also, if the patient has already received this injection, it is necessary to verify the injection site that was previously used and to ensure that the previous dose did not result in any adverse


 Nursing Skills

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Rodent Identification I

JoVE 10189

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN

A fundamental requirement of biomedical research is the proper identification of research animals. It is essential that the right animal is utilized for procedures and data collection. Laboratory mice and rats can be identified with the following permanent methods: ear tags, ear punch codes, microchip implantation, tail tattoos for adult mice, and toe tattoos for neonates. Temporary methods of dyes and marking pens can also be used for acute studies. This video covers the technical aspects of ear tagging and punching for mice and rats, as well as the benefits of each with respect to the type of research being conducted on the animals. Knowledge of the basic manual restraint techniques for each animal (covered in a separate video) is required for these identification methods to be properly accomplished.


 Lab Animal Research

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Rodent Identification II

JoVE 10182

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN

Animal records must be accurately maintained to ensure that data collection is correct. Records range from maintaining information on cage cards to having a detailed database with all of the relevant information on each animal. The primary component of recordkeeping is the individual identification of research animals. There are a variety of methods suitable for identifying mice and rats. This video describes the procedural techniques for tattooing, microchip placement, and temporary identification methods, and also explores the benefits of each.


 Lab Animal Research

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Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-Vascular Branching Morphogenesis and Immune Cell Distribution

1Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 2Earle A. Chiles Research Institute, Robert W. Franz Cancer Center, Providence Portland Medical Center

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JoVE 57406


 JoVE In-Press

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