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Point Mutation: A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair.

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

1Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 2College of Life Sciences, University of Chinese Academy of Sciences, 3School of Life Science and Technology, Shanghai Tech University, 4Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

JoVE 56844

 Genetics

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

1Molecular and Cellular Biology Department, Beckman Research Institute at the City of Hope, 2Irell & Manella Graduate School of Biological Sciences, 3Department of Molecular Immunology, Beckman Research Institute and the City of Hope

JoVE 59329

 Immunology and Infection

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

1Department of Molecular Cell Biology, University of California, Berkeley, 2Howard Hughes Medical Institute, University of California, Berkeley, 3Innovative Genomics Institute, University of California, Berkeley, 4Biomedical Sciences Graduate Program, University of California, San Francisco, 5Department of Microbiology and Immunology, University of California, San Francisco, 6Diabetes Center, University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine, University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, 10Department of Integrative Biology, University of California, Berkeley

JoVE 57350

 Genetics

Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins

1Research Institute for Biomedical Aging Research, University of Innsbruck Austria, 2Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck Austria, 3Division of Genetic Epidemiology, Medical University of Innsbruck Austria, 4Faculty of Chemistry, Department of Organic Chemistry, University of Vienna Austria

JoVE 59729

 Biochemistry

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

1State Key Laboratory of Reproductive Medicine, Nanjing Medical University, 2The Affiliated Hospital of Hangzhou Normal University, 3School of Life Science and Technology, ShanghaiTech University, 4Department of Tissue and Embryology, School of Basic Medical Sciences, Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan University

JoVE 59830

 Genetics

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

1Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, 2Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 3Oak Ridge Institute for Science and Education

JoVE 56945

 Cancer Research

Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice

1Experimental Neuropsychopharmacology, Federal Institute for Drugs and Medical Devices, 2KBRwyle GmbH, 3Cognitive Neurophysiology, Department of Psychiatry and Psychotherapy, Faculty of Medicine, University of Cologne, 4Molecular and Cellular Cognition, German Center for Neurodegenerative Diseases (DZNE), 5Institute of Neurophysiology, Faculty of Medicine, University of Cologne, 6Federal Institute for Drugs and Medical Devices (BfArM), 7Thescon GmbH

JoVE 59200

 Neuroscience

Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis

1Unit of Microscopy and Dynamic Imaging, Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Centro di Imaging Sperimentale, Ospedale San Raffaele, 3Unit of Gynecological Oncology Research, European Institute of Oncology IRCCS

Video Coming Soon

JoVE 60398

 JoVE In-Press

Databases to Efficiently Manage Medium Sized, Low Velocity, Multidimensional Data in Tissue Engineering

1Department of Biomedical Engineering, University of California, Irvine, 2The Edwards Lifesciences Center for Advanced Cardiovascular Technology, University of California, Irvine, 3Pediatrics-Genetics & Genomics Division-School of Medicine, University of California, Irvine, 4Biological Chemistry-School of Medicine, University of California, Irvine, 5Department of Chemical and Biomolecular Engineering, University of California, Irvine, 6Center for Complex Biological Systems, University of California, Irvine, 7The NSF-Simons Center for Multiscale Cell Fate Research (CMCF), University of California, Irvine

Video Coming Soon

JoVE 60038

 JoVE In-Press

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

1Laboratory of Clinical Chemistry and Hematology, University Medical Center Utrecht, Utrecht University, 2Van Creveldkliniek, University Medical Center Utrecht, Utrecht University, 3Department of Pediatrics, Division of Hematology/Oncology, Baylor College of Medicine

Video Coming Soon

JoVE 60213

 JoVE In-Press

Establishment and Characterization of Three Afatinib-resistant Lung Adenocarcinoma PC-9 Cell Lines Developed with Increasing Doses of Afatinib

1Advanced Cancer Translational Research Institute, Showa University, 2Division of Breast Surgical Oncology, Department of Surgery, Showa University School of Medicine, 3Division of Allergology and Respiratory Medicine, Department of Medicine, Showa University School of Medicine

JoVE 59473

 Cancer Research

Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella

1Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, Collaborative Innovation Center of Chemical Science and Engineering, School of Pharmaceutical Science and Technology, Tianjin University, 2State Key Laboratory of Ecological Pest Control for Fujian/Taiwan Crops and Institute of Applied Ecology, Fujian Agriculture and Forestry University, 3Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou, 4Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University

JoVE 58568

 Biochemistry
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