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Sodium Hydroxide: A highly caustic substance that is used to neutralize acids and make sodium salts. (From Merck Index, 11th ed)

Introduction to Titration

JoVE 5699

Source: Laboratory of Dr. Yee Nee Tan — Agency for Science, Technology, and Research


Titration is a common technique used to quantitatively determine the unknown concentration of an identified analyte.1-4 It is also called volumetric analysis, as the measurement of volumes is critical in titration. There are many types of titrations…

 General Chemistry

Diffusion and Osmosis - Prep Student

JoVE 10563

Preparation of Solutions for the Agar Cube Experiment
IMPORTANT: Wear gloves, goggles, and appropriate personal protective equipment – chemicals can be hazardous at high concentrations.
For the diffusion indicator solution, weigh out 1 g of phenolphthalein and add it to a beaker containing 100 mL of 95% ethanol.
To make the basic…

 Lab Bio

Using a pH Meter

JoVE 5500

Source: Laboratory of Dr. Zhongqi He - United States Department of Agriculture


Acids and bases are substances capable of donating protons (H+) and hydroxide ions (OH-), respectively. They are two extremes that describe chemicals. Mixing acids and bases can cancel out or neutralize their extreme effects. A substance that is neither acidic…

 General Chemistry

Crystallization of Salicylic Acid via Chemical Modification

JoVE 10407

Source: Kerry M. Dooley and Michael G. Benton, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA


Processing of biochemicals involves unit operations such as crystallization, ultracentrifugation, membrane filtration, and preparative chromatography, all of which have in common the need to separate large from…

 Chemical Engineering

Determining the Solubility Rules of Ionic Compounds

JoVE 10197

Source: Laboratory of Dr. Neal Abrams — SUNY College of Environmental Science and Forestry


An ionic compound's solubility can be determined via qualitative analysis. Qualitative analysis is a branch of analytical chemistry that uses chemical properties and reactions to identify the cation or anion present in a chemical compound.…

 General Chemistry

Safe Handling of Mineral Acids

JoVE 10370

Source: Robert M. Rioux & Taslima A. Zaman, Pennsylvania State University, University Park, PA


A mineral acid (or inorganic acid) is defined as a water-soluble acid derived from inorganic minerals by chemical reaction as opposed to organic acids (e.g. acetic acid, formic acid). Examples of mineral acids…

 Lab Safety

DNA Methylation Analysis

JoVE 5550

Methylation at CpG dinucleotides is a chemical modification of DNA hypothesized to play important roles in regulating gene expression. In particular, the methylation of clusters of methylation sites, called “CpG islands”, near promoters and other gene regulatory elements may contribute to the stable silencing of genes, for example, during epigenetic processes…

 Genetics

Capillary Electrophoresis (CE)

JoVE 10226

Source: Laboratory of Dr. B. Jill Venton - University of Virginia


Capillary electrophoresis (CE) is a separation technique that separates molecules in an electric field according to size and charge. CE is performed in a small glass tube called a capillary that is filled with an electrolyte solution. Analytes are separated due to…

 Analytical Chemistry

Nutrients in Aquatic Ecosystems

JoVE 10023

Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University


Nitrogen and phosphorus are essential plant nutrients found in aquatic ecosystems and both are monitored as a part of water quality testing because in excess amounts they can cause significant water quality problems. 


 Environmental Science

Tensile Strength of Resorbable Biomaterials

JoVE 10471

Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut


For over 4000 years, sutures have been used as a medical intervention. The earliest records indicate linen was the biomaterial of choice. Catgut, which is still in use today, was reportedly used to …

 Biomedical Engineering

High-Performance Liquid Chromatography (HPLC)

JoVE 10156

Source: Dr. Paul Bower - Purdue University


High-performance liquid chromatography (HPLC) is an important analytical method commonly used to separate and quantify components of liquid samples. In this technique, a solution (first phase) is pumped through a column that contains a packing of small porous particles with a second phase bound to …

 Analytical Chemistry

Spectrophotometric Determination of an Equilibrium Constant

JoVE 10094

Source: Laboratory of Dr. Michael Evans — Georgia Institute of Technology


The equilibrium constant, K, for a chemical system is the ratio of product concentrations to reactant concentrations at equilibrium, each raised to the power of their respective stoichiometric coefficients. Measurement of K involves…

 General Chemistry

Making Solutions in the Laboratory

JoVE 5030

The ability to successfully make solutions is a basic laboratory skill performed in virtually all biological and chemical experiments. A solution is a homogenous mixture of solute dissolved in bulk liquid known as the solvent. Solutions can be described by their solute concentration, a measure of how much solute is present per unit of solution.

In this…

 General Laboratory Techniques

Isolation of Fecal Bacteria from Water Samples by Filtration

JoVE 10213

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - Arizona University
Demonstrating Author: Luisa Ikner


The quality of water destined for use in agricultural, recreational, and domestic settings is of great importance due to the potential for outbreaks of waterborne disease. Microbial agents implicated in such events…

 Environmental Microbiology

Common Lab Glassware and Uses

JoVE 10161

Source: Laboratory of Dr. Neal Abrams — SUNY College of Environmental Science and Forestry


Glassware is a regular appearance in the professional chemistry laboratory, because it has a relatively low cost, extreme durability, and specific levels of precision. While some labware is being supplemented with plastic or even everyday…

 General Chemistry

Genetic Screens

JoVE 5542

Genetic screens are critical tools for defining gene function and understanding gene interactions. Screens typically involve mutating genes and then assessing the affected organisms for phenotypes of interest. The process can be “forward”, where mutations are generated randomly to identify unknown genes responsible for the phenotypes, or it can be…

 Genetics

VirWaTest, A Point-of-Use Method for the Detection of Viruses in Water Samples

1Laboratory of Viruses Contaminants of Water and Food (VIRCONT), Department of Genetics, Microbiology and Statistics, Section of Microbiology, Virology and Biotechnology, University of Barcelona, 2Grupo de Investigación Biodiversidad, Medio Ambiente y Salud (BIOMAS), Facultad de Ingenierías y Ciencias Agropecuarias (FICA), Ingeniería en Biotecnología, Universidad de las Américas, 3Municipal Laboratory - Waters of Mataró, 4GenIUL

JoVE 59463

 Immunology and Infection

Creating a Structurally Realistic Finite Element Geometric Model of a Cardiomyocyte to Study the Role of Cellular Architecture in Cardiomyocyte Systems Biology

1Cell Structure and Mechanobiology Group, University of Melbourne, 2Systems Biology Laboratory, Melbourne School of Engineering, University of Melbourne, 3Department of Biomedical Engineering, University of Melbourne, 4School of Mathematics and Statistics, Faculty of Science, University of Melbourne, 5Department of Engineering Science, University of Auckland, 6Advanced Microscopy Facility, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 7ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, 8School of Medicine, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, 9Living Systems Institute, University of Exeter

JoVE 56817

 Bioengineering

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy

1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 2Johns Hopkins Physical Sciences - Oncology Center, Johns Hopkins University, 3Department of Biomedical Engineering, Johns Hopkins University, 4Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine

JoVE 56364

 Bioengineering
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