Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Chemistry / Evaluation of Oxidative Stress in Biological…
JoVE Journal
Chemistry

A subscription to JoVE is required to view this content.

JoVE Journal Chemistry
Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Synthesis of Single-Crystalline Core-Shell Metal-Organic Frameworks
Journal (Chemistry)
Synthesis of Single-Crystalline Core-Shell Metal-Organic Frameworks
Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry
Journal (Chemistry)
Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry
 

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay

Article DOI: 10.3791/61122-v • 06:19 min • May 12th, 2020
May 12th, 2020 •
1,
1School of Molecular Sciences, The Biodesign Institute - Center for Personalized Diagnostics, Arizona State University

Chapters

Summary
Automatic Translation

The goal of the thiobarbituric acid reactive substances assay is to assess oxidative stress in biological samples by measuring the production of lipid peroxidation products, primarily malondialdehyde, using visible wavelength spectrophotometry at 532 nm. The method described here can be applied to human serum, cell lysates, and low density lipoproteins.

Tags

TBARS Assay Oxidative Stress Evaluation Biological Samples TBARS Levels Intra-laboratory Assessment Cost-effective Commercially Available Kits Batch Analysis Biospecimens Stability Studies Sample Preservation Fresh Reagents PH Control Absorbance Measurement Thiobarbituric Acid Solution Sodium Hydroxide Solution
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter