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Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice

Article DOI: 10.3791/2644-v • 07:56 min • August 4th, 2011
August 4th, 2011 •
1,2, 2,3, 2,3, 4, 2,3, *2,3,5,6, *4,5,7
1Interdepartmental Program in Translational Biology & Molecular Medicine, Baylor College of Medicine, 2Texas Children's Cancer Center, Baylor College of Medicine, 3Department of Pediatrics, Baylor College of Medicine, 4Department of Pathology, The Methodist Hospital Research Institute, 5Department of Ophthalmology, Retinoblastoma Center of Houston, 6Baylor College of Medicine, Center for Cell and Gene Therapy, 7Center for Cell and Gene Therapy, Baylor College of Medicine
* These authors contributed equally

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A method is described to propagate human retinoblastoma tumors in mice. Tumor cells are directly injected into the eyes of immune deficient mice. Secondary tumors have been successfully established using both cells directly harvested from human tumors and cultured tumorspheres.

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Establishment Propagation Human Retinoblastoma Tumors Immune Deficient Mice Cultured Tumorspheres Cellular Phenotypes New Tumors Formation Rag2-/- Immune Deficient Mice Vitreous Cavity Murine Eyes Tumorspheres In Vitro Three Passages Continuing Source Human Retinoblastoma Tissue Wesley S. Bond Lalita Wadhwa
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